Human colitis-on-chip model reveals dual roles of butyrate in epithelial and macrophage defense against Candida albicans tissue invasion
Allwang, M., Wipplinger, M., Akbarimoghaddam, P., Alonso-Román, R., Cseresnyes, Z., Dietschmann, A., Wegner, V., Feile, A., Bachelot, Y., Warschinke, M., Hassan, M.I.A., Mittag, S., Huber, O., Hube, B., Gresnigt, M.S., Figge, M.T., Mosig, A.S. (2026). Small (Weinheim, Germany) ·
DOI ↗An immunocompetent colitis-on-chip that reveals the microbial metabolite butyrate acting in two directions at once, supporting the epithelial barrier while shaping how macrophages defend against Candida albicans tissue invasion.
Secondary bile acid lithocholic acid ameliorates colitis-like inflammation in a human intestine-on-chip system
Kaden, T., Allwang, M., Stallhofer, J., Graf, K., Raasch, M., Mosig, A.S. (2026). Frontiers in Immunology ·
DOI ↗Using a human intestine-on-chip, this work shows that the secondary bile acid lithocholic acid eases colitis-like inflammation, pointing to a protective role for microbiota-derived bile acids.
Modeling Clostridioides difficile toxin pathogenesis and antiserum protection in an immunocompetent intestine-on-chip platform
Wegner, V.D., Warschinke, M., Ben Brahim, I., Feile, A., Huber, K.E., Mosig, A.S. (2026). Scientific Reports ·
DOI ↗An immunocompetent intestine-on-chip reproduces Clostridioides difficile toxin damage and is used to test antiserum protection, offering a human platform for antitoxin strategies.
Synergistic cross-kingdom host cell damage with Candida albicans relies on Enterococcus faecalis cytolysin
Kapitan, M., Niemiec, M.J., Swidergall, M., Millet, N., Brandt, P., Chowdhury, E., Hoffmann, F., Höring, S., Lange, A., Veleba, M., Nietzsche, S., Mosig, A.S., Löffler, B., Kline, K., Vylkova, S., Jacobsen, I.D. (2025). Proceedings of the National Academy of Sciences ·
DOI ↗Shows that Candida albicans and Enterococcus faecalis act together to injure host cells, with the bacterial cytolysin driving this synergistic cross-kingdom damage relevant to gut infection.
Short-chain fatty acids modulate anti-ROR1 CAR T-cell function and exhaustion in an intestinal adenocarcinoma-on-chip model
Wegner, V.D., Feile, A., Alb, M., Hudecek, M., Hewitt, P., Mosig, A.S. (2025). Advanced Healthcare Materials ·
DOI ↗In an intestinal adenocarcinoma-on-chip, short-chain fatty acids from the microbiome modulate the activity and exhaustion of anti-ROR1 CAR T cells, linking gut metabolites to cell-based cancer therapy.
Leveraging organ-on-chip models to investigate host-microbiota dynamics and targeted therapies for inflammatory bowel disease
Kaden, T., Alonso-Román, R., Stallhofer, J., Gresnigt, M.S., Hube, B., Mosig, A.S. (2025). Advanced Healthcare Materials ·
DOI ↗A review of how organ-on-chip models capture host-microbiota dynamics and support targeted therapies in inflammatory bowel disease.
Modeling of intravenous caspofungin administration using an intestine-on-chip reveals altered Candida albicans microcolonies and pathogenicity
Kaden, T.*, Alonso-Román, R.*, Akbarimoghaddam, P.*, Mosig, A.S., Graf, K., Raasch, M., Hoffmann, B., Figge, M.T., Hube, B., Gresnigt, M.S. (2024). Biomaterials ·
DOI ↗Models intravenous caspofungin treatment in an intestine-on-chip and shows how the antifungal reshapes Candida albicans microcolonies and their pathogenicity.
Immunocompetent intestine-on-chip model for analyzing gut mucosal immune responses
Feile, A., Wegner, V., Mosig, A.S. (2024). Journal of Visualized Experiments (JoVE), (207) ·
DOI ↗A step-by-step protocol for building and running the immunocompetent intestine-on-chip to analyse mucosal immune responses in the gut.
An organ-on-chip platform for simulating drug metabolism along the gut-liver axis
Lucchetti, M., Kehinde Oluwasegun, A., Grandmougin, L., Jäger, C., Perez-Escriva, P., Letellier, E., Mosig, A.S., Wilmes, P. (2024). Advanced Healthcare Materials ·
DOI ↗Couples gut and liver tissues on a single chip to reproduce drug metabolism along the gut-liver axis.
Short chain fatty acids, key regulators of the local and systemic immune response in inflammatory diseases and infections
Ney, L.M., Wipplinger, M., Grossmann, M., Engert, N., Wegner, V.D., Mosig, A.S. (2023). Open Biology ·
DOI ↗Reviews how short-chain fatty acids produced by the gut microbiota regulate local and systemic immune responses in inflammation and infection.
Intestinal stem cell-on-chip to study human host-microbiota interaction
Siwczak, F., Loffet, E., Kaminska, M., Koceva, H., Mahe, M.M., Mosig, A.S. (2021). Frontiers in Immunology ·
DOI ↗A review on intestinal stem cell-on-chip systems for studying human host-microbiota interactions.
Keeping Candida commensal, how lactobacilli antagonize pathogenicity of Candida albicans in an in vitro gut model
Graf, K., Last, A., Gratz, R., Allert, S., Linde, S., Westermann, M., Gröger, M., Mosig, A.S., Gresnigt, M.S., Hube, B. (2019). Disease Models & Mechanisms ·
DOI ↗Demonstrates in an in vitro gut model how lactobacilli keep Candida albicans in check, antagonising its switch to a pathogenic state.
A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies
Maurer, M., Gresnigt, M.S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., Cseresnyes, Z., Medyukhina, A., Graf, K., Gröger, M., Raasch, M., Siwczak, F., Nietzsche, S., Jacobsen, I.D., Figge, M.T., Hube, B., Huber, O., Mosig, A.S. (2019). Biomaterials ·
DOI ↗Introduces the three-dimensional immunocompetent intestine-on-chip that rebuilds the gut barrier together with immune cells for functional and host-microbe studies.